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細胞微流體工作站VenaFlux (VenaFlux Platforms)是Cellix公司目前*受客戶歡迎的一套系統,提供在活細胞水平上微流體活性篩選的完整解決方案。操作簡單,分析效率高,可大大提高藥品的開發和篩選效率。
VenaFlux 系統是**個能進行連續流體細胞分析的半自動化微流體分析系統。配合使用Cellix生物芯片和細胞分析軟件,VenaFlux 系統可檢測粘附到微毛細管上的細胞,微毛細管中覆蓋抗體或者培養有內皮細胞,模擬生理流動產生一定的剪切力。
VenaFlux 系統操作簡單,可減少耗時耗錢的動物模型實驗,獲得特異性強,準確性高,重復性好的結果。
VenaFlux 流體細胞分析儀產品特點:
? 廣泛使用于各種細胞懸浮液;
? 生物芯片可選擇用光學顯微鏡做細節研究;
? 微流體泵可方便的控制流體剪切力在0.05到20dyne/cm2范圍內;
? 在分析中可調整剪切力;
? 流體速度可控制在pL/min到mL/min;
? 可做nL到mL 的樣品分析;
? 使用DucoCell 分析軟件(DucoCell Analysis Software),可簡單快速的調節好顯微鏡的各項參數;
? 統計分析生成的圖表(如:細胞粘附性VS.剪切力)可方便的導入Excel文檔;
? 電子機械臂移動生物芯片,清洗細微組件和稀釋細胞樣品;
? VenaFluxTM 系統的軟件同步調節泵,翻譯階段篩選,圖像采集,樣品移動和流體控制,各項操作界面均是用戶友好型,系統先進又易于操作;
細胞微流體工作站 (VenaFlux Platforms)的主要技術參數:
Microscope Axiovert 40:
Contrast option: Brightfield, Phase, Varel.
Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan
Eyepieces: E-PL 10x/20 Br., 10x/20 Br. foc.
Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.
Mirus 1.0 Nanopump
Specifications: Shear Stress Range of 0.05–20 dyne/cm2,
Volumetric Flow Rates 100nL–20μL/minute (100μL syringe),
Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s;
Flow Direction Reversible;
Sample Volume Aspiration Accuracy ±1% ;
Shear Stress Accuracy ±0.5%;
Sample Volume Aspiration Precision < 1%.
Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.
Microscope Cage incubator:
Capabilities: Temperature control ± 0.10C, CO2 and humidity module.
Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.
Deltapix DP 200 USB 2.0 camera
Specifications: 1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2" format, Pixel 5.2 μm x 5.2 μm,
Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.
Dell Optiplex 745 PC computer
Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;
Objective Imaging stage control hardware; 19” LCD monitor.
Windows XP Professional SP2;
DeltaPix Viewer Software;
FlowAssay and DucoCell software preinstalled.
FlowAssay Software.
Capabilities: Execution of preset protocols.
Intuitive stepwise protocol interface with steps hierarchy and status control.
DucoCell software
Specifications: Automatic Counting and Detection;
Morphological parameters analyzed automatically(Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis);
Cell sorting and filtering;
Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.
Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).
File Formats analyzed*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.
Vena8 biochips pack of 10
Specifications: Minimum Sample Volume 3 μL;
Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2.
Number of channels per biochip is 8;
Volume of each channel is 0.8μL.
Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).
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