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儀器簡介:快速動力學停流裝置與光譜儀器配合進行快速動力學瞬間實時記錄,或離線分析,為廣大的快速動力學研究學者提供了強有力的武器。選擇不同的配置可進行下列應用:
* 蛋白質折疊動力學(Protein Folding)
* 酶動力學(Enzyme Kinetice)
* 底物結合(Substrate Binding)
* 構象轉變(Conformational Changes)
* 胞內底物運輸(Substrate Transport in Vesicles)
* 第二信使研究(Second Messenger Studies)
* 快速化學反應動力學(Rapid Chemical Reaction Kinetics)
KinTek has always been aleader in building stopped-flow instrumentsthat conserve precious samples. The all new AutoSF-120 sets a new standard for sample economy, requiringonly 120 microliters of sample to flush, load and collect data, while giving with 3-4 replicate measurements.The flushing and sample loading areall done automatically. Simply pipette 120 microliters of solution into each sample cup and click "Load".
In the process of designing this new state-of-the-art instrument, we also added new noise reduction electronic circuits to improve signal:noise twofold and achieved asubmillisecond dead time. In addition we completely re-wrote the system software for data collection and analysis to give unparalleled ease of use and power.
The KinTek AutoSF is by far the best stopped-flow instrument ever built, giving you more high quality data while consumingless sample and less time.
An optionalrobotic autosamplerincreases throughput even more to afford unattended stopped-flow measurements ofhundreds of samples per day.
技術參數:
1.注射器數量:3個
2.驅動方式:每一個注射器由一個步進馬達驅動
3.*小死時間:0.6毫秒(FC-08池)0.25毫秒(微量池)
4.注射流量范圍:0.01-10ml/s(取決于安裝的注射器)
5.混合比例范圍:1/1至1/100連續可調
主要特點:
1.恒溫水夾套設計,全電腦控制操作
2.程控驅動注射器,每路樣品流量可調,不受濃度與粘度影響
3.高精度,完全可調的混合與稀釋比例
4.極短的死時間,*短為0.25毫秒
5.二路、三路、四路流動注射停流裝置可選擇
Small sample volume:120 μL is sufficientto flush and load a new sample and get3-4 reproducible shots.All other instruments require 3-5 times as much sample.
Automated computer-controlledload/flush/fire cycle — simply pipet 120 μL into each sample cup and click "load".
Twofold improvement in signal/noise with new noise reduction circuits
Compact footprint: instrument and electronics combined (14 × 26.5 × 4.5 inches)
Computer optimized optical path with lower noise and background fluorescence
Automated sample loader, which can be interfaced with an optional robot.
200 μl of sample is sufficient to load and collect 6-8 good shots.
Standard 10 μl flow cell.
20 μl per shot per solution.
Hastwo fluorescence channelsandone absorbance channelthat can be observed and recorded simultaneously.
Under complete computer control, courtesy of the KinTek StopFlow Software.